Revisiting overexpression of a heterologous β-glucosidase in Trichoderma reesei: fusion expression of the Neosartorya fischeri Bgl3A to cbh1 enhances the overall as well as individual cellulase activities
Xianli Xue, Yilan Wu, Xing Qin, Rui Ma, Huiying Luo, Xiaoyun SuEmail author and Bin YaoEmail author
The filamentous fungus Trichoderma reesei has the capacity to secret large amounts of cellulase and is widely used in a variety of industries. However, the T. reesei cellulase is weak in β-glucosidase activity, which results in accumulation of cellobiose inhibiting the endo- and exo-cellulases. By expressing an exogenous β-glucosidase gene, the recombinant T. reesei cellulase is expected to degrade cellulose into glucose more efficiently.
http://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-016-0520-9
