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Anaspec introduces the release of a recombinant http://www.teamskyuser.co.uk/beats-by-solo-diamond.html human baff protein


Anaspec is pleased to announce the release of a recombinant human beats by dre sale baff protein.Nave bcells, treated with 201000 ng/ml baff protein prepared by anaspec, has been showing increased igg secretion compared to 2 leading commercial sources.

Baff(Blys, tall1, thank, tnfsf13b, ztnf4)Belongs to the tnf superfamily of proteins and is expressed by myeloid cells.14 It is a 285amino acid long type II transmembrane protein, with the soluble152amino acid active region.14 Baffplays a pivotal role in Bcells homeostasis including survival of immature Bcells, transition of Blymphocytes from T1 to T2 stage, and causes increase of antibodies secretion by Bcells.14

The sequence(Accession np_006564)Corresponding to the extracellular domain of human baffAlong with a 6x his tag was expressed in e.Coli.Recombinant human baff was purified from urea denatured bacterial lysate using immobilized metal affinity chromatography(Imac)And refolded into its active state using a proprietary technique.The molecular weight of the recombinant human baff is17.2 kDa.Containing less than 0.1 EU of endotoxin per1 g of protein as determined by LAL assay, the activity of BAFF was determined by its ability to stimulate production of immunoglobulin molecules such as IgG, IgA, and IgM in isolated mouse Blymphocytes.Purified recombinant human baff is supplied as sterile and frozen condition at 0.1 mg/ml in1 x PBS buffer with5 mM DTT, pH=7.5.1.Batten, m.Et.Al. (2000) J Exp Med192 (10):14531465

2.Parry, tj.Et.Al. (2001)J pharm exp ther 296(2):396404

3.Shan, x.Et.Al. (2006)Physiol res 55:301307

4.Katsenelson, n.Et.Al. (2007)Eur j immunol 37:17851795AnaSpec is a leading provider of integrated proteomics solutions to the world's largest biotech, pharmaceutical, and academic research institutions.With a vision for innovation through synergy, anaspec focuses on three core technologies:Peptides, detection reagents, and antibodies.

Anaspec recently joined forces with eurogentec to provide an even broader spectrum of products and services to serve the worldwide life science community.Eurogentec employs 400+ people globally.



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